Tag
Part:BBa_K3815025:Design
Designed by: Alexander Liu Group: iGEM21_Kyoto (2021-10-20)
AANDENYATAP. mutant SsrA degradation tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part can be fused to the C-terminus of your proteins of interest to promote their degradation. Note that you do not have to add stop codons because the part includes TAATAA at the 3' end.
Source
Escherichia coli